Reconstitution and purification of flagellar filaments from Caulobacter crescentus.
نویسندگان
چکیده
Filaments from isolated flagella of Caulobacter crescentus have been purified by successive dissociation and reconstitution. After the second and third reconstitutions from subunits in 0.8 M sodium citrate, filament preparations contained only two proteins, flagellin A (26,000 daltons) and flagellin B (28,000 daltons). There was some enrichment for flagellin A during reconstitution by this procedure, since isolated flagella contained flagellin A and flagellin B in a ratio of approximately 3.8:1 and filaments after the third reconstitution contained the two proteins in a ratio of 5.0:1.
منابع مشابه
A family of six flagellin genes contributes to the Caulobacter crescentus flagellar filament.
The Caulobacter crescentus flagellar filament is assembled from multiple flagellin proteins that are encoded by six genes. The amino acid sequences of the FljJ and FljL flagellins are divergent from those of the other four flagellins. Since these flagellins are the first to be assembled in the flagellar filament, one or both might have specialized to facilitate the initiation of filament assembly.
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 132 3 شماره
صفحات -
تاریخ انتشار 1977